Piperine, an alkaloid that gives black pepper its pungent taste (Piper nigrum), is also associated with several bioactivities and can be used as a spray in personal defense devices. The preparative separation and purification of piperine is usually performed by conventional methods. Among those methods are centrifugal partition chromatography (CPC) and flash chromatography. In this study, a crude extract of black pepper is used to purify piperine and to compare the performance of CPC versus flash chromatography - read more about the methods and results in this application note.
In recent years, kratom has become an increasingly popular recreational drug around the world; consequently, this increase in popularity is matched by increasing concern over its potential to worsen the current opioid epidemic. As a result, further studies of kratom and its constituent compounds are necessary to better understand the mechanisms of action of these compounds and to evaluate possible therapeutic benefits of mitragynine or 7-hydroxymitragynine, present in kratom. Learn more in this application note.
Using the right systems can improve sample integrity in your sample separation workflows, helping labs to have greater confidence in their results. Here we outline what to look for in your sample separation instruments to remove risks of common threats to sample integrity.
In this video, you will learn about the benefits of Centrifugal Partition Chromatography (CPC) as it relates to other forms of traditional sample separation. Without solid support, liquid-liquid chromatography allows for repeat injections of samples with 100% recovery of the materials--and you'll never need to replace any silica columns. CPC columns tackle tough separations of complex samples in a single step without prior purification, making it an ideal choice for natural product isolation, protein purification, and more.
While more than 220 viruses are known to infect humans, clinically approved antiviral drugs are available for only a dozen of these viruses. CPC is an ideal solution for natural compound investigations. Gilson, in collaboration with Brazilian researchers, recently performed preliminary experiments demonstrating the antiviral activity of compounds that were fractionated from a plant crude extract using CPC. Read more about the study here.
In this article, we hear from an industry leader on their experience using centrifugal partition chromatography (CPC) in the pharmaceutical industry, and how it's a method well-suited for producing highly active molecules that meet safety and performance requirements.
Lignin is an abundant phenylpropanoid polymer found in all plant matter. It has historically been treated as an impurity in the paper pulping and ethanol biorefinery industries where it is burned as a low-value fuel or sold at low prices. However, developing higher-value applications of lignin would help biorefineries operate at higher efficiencies. In this app note, a two-dimensional separation is conducted using centrifugal partition chromatography (CPC), which helps demonstrate the value of CPC as a tool for gaining deeper insights into depolymerization mixtures for biomass facilities.
Forsythiaside A, a phenylethanoid glycoside, is one of the major active constituents extracted from the air-dried fruits of Forsythia suspensa and has been reported to possess a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities. This application note discusses a simple, fast, and cost-efficient method to prepare pure compounds using a centrifugal partition chromatography (CPC) system.
Whether you’re purifying at small-scale preclinical research levels or larger manufacturing levels, Gilson’s scalable lineup of high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC) systems has you covered. These solutions are dependable, flexible, and precise. Our team of experts is here to help you find the one that is right for you.
The rise of biorenewable alternatives and petroleum/fossil-derived chemicals have led to a growing interest in lignin valorization. In this webinar, Gilson's Dr. Manar Alherech demonstrates the feasibility of isolating valuable compounds from an oxidative lignin depolymerization matrix using centrifugal partition chromatography (CPC).